The ISIS vault is a room located in ISIS headquarters. The entrance is secured within the same room as the ISIS mainframe. It holds the backup servers and other miscellaneous items. It is secured with an extremely tough metal and security lock.
- In "A Going Concern," Barry and Lana are lured here so the ISIS employees can trap them while they sabotage Len Trexler's plan to buy ISIS and make it part of ODIN.
- In "Tragical History," Lana, Archer, Malory and Doctor Krieger attempt to get into the vault to turn off the backup server. Unfortunately the server that opens the door is infected by the virus so they can't access it.